Anti-Human selectin P Recombinant Antibody (Inclacumab)
CAT#: TAB-246
Recombinant monoclonal antibody to selectin P. Inclacumab (INN) is a humanized monoclonal that binds to selectin P.
Published Data
Tested Data
Gene Expression
Inhib
Figure 1 Inhibition of TRAP-induced PLAs and PMAs in human blood.
Anti-coagulated human blood was spiked with the indicated inclacumab concentrations prior to TRAP activation. Inhibition of PLAs (A) or PMAs (B) is given in % of the TRAP-induced increase of PLAs or PMAs from non-activated baseline levels. Values represent means ± SEM (n = 3). The dotted line indicates the IC50 value.
Kling, D., Stucki, C., Kronenberg, S., Tuerck, D., Rhéaume, E., Tardif, J. C.,... & Schmitt, C. (2013). Pharmacological control of platelet-leukocyte interactions by the human anti-P-selectin antibody inclacumab–preclinical and clinical studies. Thrombosis research, 131(5), 401-410.
Agonist
Figure 2 Impact of inclacumab on PLAs induced by various platelet agonists.
Inclacumab (10 μg/mL) was added to anti-coagulated human blood prior to activation with TRAP (A), ADP (B), collagen (C) and AA (D). Maximal concentrations of TRAP (2 μM), ADP (5 μM), collagen (10 μg/mL) and AA (0.5mg/mL) resulted in ~70%, 40%, 10% and 20% P-selectinpositive platelets, respectively (data not shown). Values represent means ± SEM (n = 3).
Kling, D., Stucki, C., Kronenberg, S., Tuerck, D., Rhéaume, E., Tardif, J. C.,... & Schmitt, C. (2013). Pharmacological control of platelet-leukocyte interactions by the human anti-P-selectin antibody inclacumab–preclinical and clinical studies. Thrombosis research, 131(5), 401-410.
Activ
Figure 3 Surface expression of the active form of CD11b on platelet-bound leukocytes.
Inclacumab was added to anti-coagulated human blood prior to activation with TRAP (25 μM). Activation of CD11b was detected on CD61+ CD45+-leukocytes using the CBRM1/5-PE mAb. Bars represent MFI of the CBRM1/5-PE binding expressed in percent of the TRAP-activated control (represented as 100%). Values represent means ± SEM (n = 3).
Kling, D., Stucki, C., Kronenberg, S., Tuerck, D., Rhéaume, E., Tardif, J. C.,... & Schmitt, C. (2013). Pharmacological control of platelet-leukocyte interactions by the human anti-P-selectin antibody inclacumab–preclinical and clinical studies. Thrombosis research, 131(5), 401-410.
Inhib
Figure 4 Inhibition of leukocyte adhesion to platelet monolayer in an ex vivo human flow system.
Human blood drawn from healthy subjects was perfused over collagencoated coverslips in the presence and absence of inclacumab. Each concentration was tested using blood from ≥4 human subjects (number of subjects is given on top of bars). Inhibition of leukocyte adhesion is given in % of the control adhesion per donor. Values represent means ± SEM.
Kling, D., Stucki, C., Kronenberg, S., Tuerck, D., Rhéaume, E., Tardif, J. C.,... & Schmitt, C. (2013). Pharmacological control of platelet-leukocyte interactions by the human anti-P-selectin antibody inclacumab–preclinical and clinical studies. Thrombosis research, 131(5), 401-410.
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Dot Blot
Figure 1 Selectin P Recombinant Antibody (TAB-246) in Dot Blot
Dot Blot analysis of TAB-246 was performed by coating with human Selectin P antigen.
TAB-246 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
ELISA
Figure 2 Selectin P Recombinant Antibody (TAB-246) in ELISA
ELISA analysis of TAB-246 was performed by coating with recombinant human Selectin P antigen.
The HRP-conjugated goat anti-human IgG as a secondary antibody.
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Normal Tissue
Figure 1 Colon
Endothelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2145/5903_A_9_3.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (EFO-21, HEL)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000174175-SELP
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Specifications
- Immunogen
- CD62P transfected 300.19 cells (Human)
- Host Species
- Human
- Derivation
- Human
- Type
- IgG4 - kappa
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- ELISA, FC, IP, FuncS, IF, Neut, ICC, Inhib, Agonist, Activ
- CAS
- 1256258-86-2
- Generic Name
- Inclacumab
- Related Disease
- Myocardial infection
Product Property
- Purity
- >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Storage
- 4°C. For long term storage, aliquot and store at -20°C. Repeated thawing and freezing must be avoided.
Applications
- Application Notes
- The SELP antibody has been reported in applications of ELISA, FC, IP, FuncS, IF, Neut, ICC, Inhib, Agonist, Activ.
Target
- Alternative Names
- Inclacumab;1256258-86-2;RG1512;R1512;RO4905417;LC1004-002;SELP;selectin P (granule membrane protein 140kDa, antigen CD62);GRMP, selectin P (granule membrane protein 140kD, antigen CD62);P-selectin;CD62;CD62P;GMP140;PADGEM;PSEL;GMP-140;granule membrane pro
- Gene ID
- 6403
- UniProt ID
- P16109
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(Creative Biolabs Cat# TAB-246, RRID: AB_3111897)
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Biosimilar Overview
Please refer to Inclacumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Inclacumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Inclacumab"
Afuco™ Anti-SELP ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-246)This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to selectin P. It is a humanized monoclonal that binds to selectin P.
DrugMonitor™ Anti-Inclacumab Antibody (VS-1224-YC598)Inclacumab is a fully human IgG4 monoclonal antibody targeting P-selectin, studied in trials for myocardial infarction, peripheral arterial disease (PAD), coronary heart disease, graft occlusion, and vascular conditions. The DrugMonitor™ Anti-Inclacumab Antibody (VS-1224-YC598) is an anti-drug antibody (ADA) against Inclacumab. This drug-based antibody is raised in mice immunized with the Inclacumab. The anti-Inclacumab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Inclacumab in samples.
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| HPAB-AP434-YC | Human Anti-SELP Recombinant Antibody (clone HuMab 003) | ELISA | Human IgG |
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